Seeking to rescue those under the stress of IB

Wednesday, 19 March 2014

4.2 Meiosis

Meiosis
Meiosis is a reduction division of a diploid nucleus to form haploid nuclei.

Homologous chromosomes definition

Homologous chromosomes - chromosomes that have the same genes in the same sequence, but not necessarily the same alleles of the genes.

Process of meiosis
Meiosis I
Prophase I
Chromosomes shorten/thicken.
Homologous chromosomes pair by synapsis (forming bivalent), chromosomes have 2 chromatids.
Nuclear envelope disintegrates.
Spindle microtubule forms.
Crossing over occurs where there is exchange of genetic material between non-sister chromatids (chiasma - point of crossover).

Metaphase I
Bivalents are attached to individual microtubules of spindle by centromeres.
Chromosomes move to equator of cell.
Orientation of maternal and paternal chromosomes are random.

Anaphase I
Homologous pairs separate; one chromosome moves to opposite poles
Pieces of chromatids exchanged during crossover

Telophase I
Chromosomes uncoil, during interphase that follows, no replication occurs; spindle breaks down.
Reduction of chromosome numbers from diploid to haploid completed.
Nuclear envelope forms, nucleolus forms, cleavage furrow forms.
Cytokinesis occurs.


Prophase II
Chromosomes condense.
Centrioles move to opposite poles.
Spindle microtubules form at right angle to previous spindle.
Nuclear envelope disintegrates.

Metaphase II
Chromosomes line up along equator.

Anaphase II
Centromeres separate / Chromatids move to opposite poles.
Daughter chromosomes separate.

Telophase II
Chromatids reach opposite poles.
Nuclear envelope forms.
4 cells with ½ chromosome number of original parent cell.
Chromatids are recognized as chromosomes, they uncoil and disperse.
Cytokinesis occurs.

Non-disjunction and Down syndrome
Non-disjunction occurs when either at anaphase I or II, the chromosomes fail to separate and go to opposite side of the poles. This leads to a gamete that has either 45 or 47 chromosomes, resulting in Down syndrome. Failing to separate during anaphase I will result in four affected daughter cells and during anaphase II, non-disjunction will lead to two affected daughter cells. For Down syndrome, one parent can contribute 2 copies of chromosomes 21 and the other can contribute 1 healthy copy, and when gametes fuse, there are 3 copies.

Non-disjunction (lc.wnlsd.ca)
 










 



Karyotyping
In karyotyping, chromosomes are arranged in pairs according to their size and structure.

Karyotyping in chorionic villus sampling and amniocentesis
Karyotyping is performed using cells collected by chorionic villus sampling or amniocentesis, for pre-natal diagnosis of chromosome abnormalities.
Amniocentesis - drawing needle, guided by ultrasound, to withdraw sample of amniotic fluid from amniotic sac.
Chorionic villus sampling - sample cells from placenta, chorion. 

Analysis of human karyotype to determine gender and non-disjunction
Male Karyotype (science-esl.wikispaces.com)
 



















Female Karyotype (daviddarling.info)

Non-disjunction Karyotype (php.med.unsw.edu.au)



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